Portfolio Update

HENDERSON INVESTMENT FUNDS LIMITED

HENDERSON ALTERNATIVE STRATEGIES TRUST PLC

Legal Entity Identifier: 213800J6LLOCA3CUDF69

Portfolio Update

In accordance with Listing Rule 15.6.8 the Company discloses that as at31 March 2017 it held the following investments in other listed closed-ended investment funds which do not have a stated investment policy to invest no more than 15% of their total assets in other listed closed-ended investment funds:

Holding % of the Company's gross assets

Riverstone Energy Limited 4.33

Henderson Secretarial Services Limited

Corporate Secretary to Henderson Alternative Strategies Trust plc